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Each of the given reactions is a coupled reaction. The first one is the reduction of cyt c (Fe3+) to cyt c (Fe2+) coupled with the oxidation of NADH to NAD+. The second is the oxidation of cyt a (Fe2+) to cyt a (Fe3+) coupled with the reduction of cyt b (Fe3+) to cyt b (Fe2+). Remember that a positive overall potential, ε'º, for the coupled reaction would make this overall reaction spontaneous.
Under standard conditions, will the following reactions proceed spontaneously as written?
a. 2Cytochrome c (Fe3+) + NADH ⇌ 2 Cytochrome c (Fe2+) + NAD+
b. Cyto a (Fe2+) + cyto b (Fe3+) ⇌ Cyto a (Fe3+) + cyto b (Fe2+)
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Based on our data, we think this problem is relevant for Professor Yeargain's class at UCF.