A single, 18-amino acid peptide chain is to be assigned from the following experimental information below, your job is to provide the full triple lettercode sequence (if possible) and show your logic behind your answer.
(i) Amino acid analysis of the native peptide: Cx6, Rx2, Ex1, Yx1, Px1, Vx1, Ax1, Mx1, Wx1, Lx1, Kx1, and Tx1.
(ii) Dinitrofluorobenzene (FDNB) analysis indicated the presence of C on reaction of the native peptide.
(iii) The native peptide is subjected to DTT and Pepsin Digestion of the native peptide produced 4 peptide fragments that were then exposed to Edman degradation analysis, they provided the following sequence information:
Fragment 1: YVPRMAC Fragment 2: CC Fragment 3: LCKETC Fragment 4: WRC
(iv) Following disulfide bond reduction, Cyanogen Bromide digestion of the original intact peptide provided 2 peptide, one 6mer + homoserine lactone and one 11mer.
(v) The resultin 11mer was HPLC isolated and exposed to TCEP, this peptide was subjected to Trypsin digestion followed by amino acid analysis. The Trypsin digested peptide fragments: A, B, andC, were assigned the following amino acid composition:
Fragment A: Cx2, Lx1, Kx1 Fragment B: Tx1, Ex1, Cx1 Fragment C: Cx1, Wx1, Ax1, Rx1