DNA Replication Video Lessons

Video Thumbnail

Concept

Problem: if Meselson and Stahl had first grown the cells in 14N-containing medium and then moved them into 15N-containing medium before taking samples, what would have been the result after each of the two replications?

FREE Expert Solution

Meselson and Stahl came up with an experiment to deduce the mechanism for DNA replication. They cultured E.coli in a medium containing nucleotide precursors labeled with 15N and transfer them to a medium containing 14N before taking samples after the first and second replication. 

If the culture was first grown with th lighter isotope (14N) isotope before being transferred to the heavier (15N) isotope prior to sample collection, the result of the first replication would be a DNA with a 50:50 hybrid containing both isotopes (14N-15N), just like that of the original experiment.

View Complete Written Solution
Problem Details

if Meselson and Stahl had first grown the cells in 14N-containing medium and then moved them into 15N-containing medium before taking samples, what would have been the result after each of the two replications?


Frequently Asked Questions

What scientific concept do you need to know in order to solve this problem?

Our tutors have indicated that to solve this problem you will need to apply the DNA Replication concept. You can view video lessons to learn DNA Replication. Or if you need more DNA Replication practice, you can also practice DNA Replication practice problems.

What professor is this problem relevant for?

Based on our data, we think this problem is relevant for Professor Thomas' class at UCF.