The 5′-end or "five prime end" designates the end of the DNA or RNA strand that has the fifth carbon in the sugar-ring of the deoxyribose or ribose at its end. A phosphate group attached to the 5′-end permits ligation of two nucleotides. On the other hand, the 3’-end is named because it is terminating at the hydroxyl group of the third carbon in the sugar-ring and is commonly known as the tail end.
Describe the difference between the 5' and 3' ends of a single-stranded nucleic acid and describe the ‘antiparallel' nature of double-stranded DNA.
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