Biology Practice Problems PCR and Dideoxy Sequencing Practice Problems Solution: Polymerase chain reaction is known for its power o...

Solution: Polymerase chain reaction is known for its power of amplifying a target DNA sequence at a high speed. Each cycle can double the number of DNA molecules (target sequence). Which of the following is CORRECT regarding PCR?A. In order to make 10 copies of the DNA, you need at least 5 cycles of PCR.B. Helicase is required in order to separate the two strands in PCR.C. Dideoxynucleotides are used in PCR.D. DNA primers are needed in PCR.E. All of the above

Problem

Polymerase chain reaction is known for its power of amplifying a target DNA sequence at a high speed. Each cycle can double the number of DNA molecules (target sequence). Which of the following is CORRECT regarding PCR?

A. In order to make 10 copies of the DNA, you need at least 5 cycles of PCR.

B. Helicase is required in order to separate the two strands in PCR.

C. Dideoxynucleotides are used in PCR.

D. DNA primers are needed in PCR.

E. All of the above

Solution

A is wrong. Creating at least 10 copies of the DNA target sequence only needs 4 PCR cycles, as long as the sample is the target sequence already.

B is wrong. The denaturation step is the one responsible for separating the two strands in PCR.

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