In the shotgun approach to whole-genome sequencing (shotgun sequencing), random DNA fragments of a chromosome are sequenced. The fragment sequences are then assembled into a continuous sequence that represents the DNA of the entire chromosome.
What are the steps in the shotgun approach to whole-genome sequencing?
Put the following in order from 1-5 (there will be one that is not used)
- multiple copies of the same chromosome are prepared
- the plasmids are sequenced
- 1-kb fragments are transcribed into RNA
- Chromosome copies are broken into 1-kb fragments
- A computer combines the fragment sequences
- 1 kb fragments are cloned into plasmids