PCR and Dideoxy Sequencing Video Lessons

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Problem: In the shotgun approach to whole-genome sequencing (shotgun sequencing), random DNA fragments of a chromosome are sequenced. The fragment sequences are then assembled into a continuous sequence that represents the DNA of the entire chromosome.What are the steps in the shotgun approach to whole-genome sequencing?Put the following in order from 1-5 (there will be one that is not used)- multiple copies of the same chromosome are prepared- the plasmids are sequenced- 1-kb fragments are transcribed into RNA- Chromosome copies are broken into 1-kb fragments- A computer combines the fragment sequences- 1 kb fragments are cloned into plasmids

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Problem Details

In the shotgun approach to whole-genome sequencing (shotgun sequencing), random DNA fragments of a chromosome are sequenced. The fragment sequences are then assembled into a continuous sequence that represents the DNA of the entire chromosome.

What are the steps in the shotgun approach to whole-genome sequencing?
Put the following in order from 1-5 (there will be one that is not used)

- multiple copies of the same chromosome are prepared

- the plasmids are sequenced

- 1-kb fragments are transcribed into RNA

- Chromosome copies are broken into 1-kb fragments

- A computer combines the fragment sequences

- 1 kb fragments are cloned into plasmids

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