Which of the following mutations would be most likely to have a harmful effect on an organism?
a. a deletion of three nucleotides near the middle of a gene
b. a single nucleotide insertion downstream of, and close to, the start of the coding sequence
c. a single nucleotide deletion near the end of the coding sequence
d. a single nucleotide deletion in the middle of an intron
e. a base-pair substitution
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