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Problem: In Eukaryotes, what are the 3 modifications between the initial transcription of a premRNA and the time it leaves the nucleus. What are they?

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Pre-mRNA is the initial RNA transcript from any gene prior to processing. Three major modifications occur before it can leave the nucleus and be translated into a protein in eukaryotes – the capping, poly(A)-tail, and splicing. 

  • Capping - An altered nucleotide is attached at the 5' end of the mRNA. This occurs to regulate nuclear export, promote translation and promote 5’ proximal intron excision. 
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In Eukaryotes, what are the 3 modifications between the initial transcription of a premRNA and the time it leaves the nucleus. What are they?

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