During the PCR, the hydrogen bonds of the double-stranded DNA molecules are broken by the enzyme helicase.
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Our tutors have indicated that to solve this problem you will need to apply the PCR and Dideoxy Sequencing concept. You can view video lessons to learn PCR and Dideoxy Sequencing. Or if you need more PCR and Dideoxy Sequencing practice, you can also practice PCR and Dideoxy Sequencing practice problems.
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Based on our data, we think this problem is relevant for Professor Yan & Burleigh & Bond's class at UF.