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No O2 was necessary for the citric acid cycle.
During oxygen debt, why would muscle cells choose lactic acid fermentation (which produces no ATP) over sending the pyruvate molecules to the citric acid cycle (2 more ATP molecules)?
a. Muscle cells do not have mitochondria
b. The ATP produced in the citric acid cycle cannot be used for cellular work
c. The production of CO2 in the citric acid cycle is detrimental to the cell
d. The citric acid cycle does not provide a mechanism for oxidizing NADH back to NAD+
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Based on our data, we think this problem is relevant for Professor Marks' class at FSU.