Concept: Nucleic Acids11m
Concept: Secondary and Tertiary Structure9m
Problem: The three components of a nucleotide are:
Problem: Uracil is used in ___________ in place of _______________________.1m
Problem: Which of the following pieces of DNA is complementary to this one 5’-ATCGAGTGA-3’ ?1m
Problem: Which piece of DNA would required the most energy to pull apart?1m
Problem: What is the secondary structure of DNA called?1m
The presence of a 2'-OH group indicates that the pentose sugar in a nucleotide is ______ while the absence of a 2'-OH group indicates the sugar in the nucleotide is _____.
You sequenced three DNA strands that are fragments of a single gene. Manually assemble the fragments and write down the complete (full) sequence.
Fragment 1: 5' - CCATGTCAGGGA - 3'
Fragment 2: 3' - TTAATTTAGGGA - 5'
Fragment 3: 5' - TAACCCCCATG - 3'
What is the reverse complement of the following DNA sequence? Give your answer in the 5' to 3' direction.
5' - TAAACAAAGAAGTACAACAA - 3'
What is the complementary DNA strand of the sequence 5' - AGTCTGAC - 3'?
If one strand of a DNA double helix has the sequence AGTACTG, what will be the sequence of the other strand?
Which of the following molecules is ribose?
Which of the following molecules is phosphate?
Which of the following is a nitrogenous base known as a purine?
Which of the following DNA double helices would be more difficult to separate into single-stranded molecules by treatment with heat, which breaks hydrogen bonds?
Explain your choice.
Identify the key structural features of a DNA molecule. Mark all that apply:
A. DNA strands are antiparallel and include a 5' end and a 3' end.
B. Strong ionic bonds and hydrophobic interaction hold DNA together.
C. DNA contains the nucleotide bases adenine, thymine, guanine, cytosine, and uracil.
D. DNA is most often found as a left-handed helix, commonly referred to as Z-DNA.
E. The backbone of DNA is made of a sugar and a phosphate molecule.
F. DNA bases are always paired purine with pyrimidine.
Which of the following DNA molecules is the most stable?
What is the complementary DNA sequence to 5′ ATGCATCG 3′? Enter only the nucleotides, and put your answer in the 5’ to 3’ direction.
If 5′ CGATGCAT 3′ is the template DNA strand, what is the sequence of the non-coding strand? Enter only the nucleotides, and put your answer in the 5’ to 3’ direction.
If 5′ CGATGCAT 3′ is the template DNA strand, what is the sequence of the mRNA transcript? Enter only the nucleotides, and put your answer in the 5’ to 3’ direction.
The template strand of a molecule of DNA contains 22%C, 18%G, 32%A, 28%T.
a. What percentage of the non-template strand is C?
b. What percentage of the RNA transcript is G?
c. What percentage of the RNA transcript is T?
Which of the following statements about DNA and RNA is FALSE?
a. DNA forms a double-stranded helical structure that contains base pairs whereas RNA is single stranded and does not form base pairs or adopt a double helical conformation.
b. DNA and RNA are both synthesized in the 5' to 3' direction.
c. DNA serves as the template for the synthesis of DNA during DNA replication and for RNA during transcription.
d. The energy for both DNA and RNA synthesis comes from cleavage of phosphoanhydride bonds in the deoxynucleotide or nucleotide building blocks.
e. DNA contains the bases A, G, C and T, whereas RNA contains the bases A, G, C and U.
How many hydrogen bonds exist between this DNA strand and its complementary strand?
5'- CATAGGA- 3'
What bonds form between complementary base pairs? Between a base and the deoxyribose sugar?
How are the monomers in nucleic acids joined?
a. Peptide bonds between carbohydrates
b. Peptide bonds between amino acids
c. Phosphodiester bonds between amino acids
d. Peptide bonds between nucleotides
e. Phosphodiester bonds between nucleotides
RNA differs from DNA in that:
a. RNA contains ribose
b. RNA contains uracil
c. All are correct
d. RNA is usually single stranded
e. RNA can catalyze chemical reactions
The rungs of the DNA ladder:
a. Are formed by base pairs joined by covalent bonds
b. Are formed by base pairs joined by hydrogen bonds
c. Are formed by base pairs joined by phosphodiester bonds
The twisted ladder of DNA is composed of building blocks called:
a. Amino acids
The 3' and 5' designations refer to the numbers that chemists assign to the:
a. Hydrogen atoms of deoxyribose
b. Oxygen atoms of deoxyribose
c. Carbon atoms in the nitrogen bases
d. Nitrogen atoms in the nitrogen bases
e. Carbon atoms of deoxyribose
Purine bases have a _________ ___________ structure:
a. Single, ring
b. Single, triangle
c. Double, triangle
d. Double, ring
e. Triple, ring
Pyrimidine bases have a __________ __________ structure:
a. Single, ring
b. Single, triangle
c. Double, ring
d. Double, triangle
e. Triple, ring
The DNA nitrogen bases that are purines are:
a. Adenine and thymine
b. Adenine and uracil
c. Guanine and thymine
d. Guanine and cytosine
e. Adenine and guanine
The DNA nitrogen bases that are pyrimidines are:
a. Cytosine and guanine
b. Uracil and cytosine
c. Thymine and cytosine
d. Thymine and adenine
e. Uracil and thymine
Strands of DNA are joined by:
a. Hydrogen bonds
b. Covalent bonds
c. Ionic bonds
d. Phosphodiester bond
DNA's sugar-phosphate backbones are joined with:
a. Ionic bonds
b. Hydrogen bonds
c. Weak chemical bonds
d. Covalent bonds
If you have 56 total nucleotides and 13 are C, how many are G?
e. none of these answers is correct.
If cytosine makes up 22% of the nucleotides in a DNA sample, then adenine would make up what percent of the bases?
e. none of these answers are correct
Which of the following is not found in RNA?
Which of the following is not a component of nucleic acids?
a. a five-carbon sugar
b. a six-carbon sugar
c. a phosphate group
d. phosphodiester bonds
e. an organic nitrogen containing base
If a double-stranded DNA sample were composed of 10% thymine, what would be the percentage of guanine?
A single strand of DNA contains the following sequence: 5'-GATTCGTCAACTG-3', the complementary single strand is:
Which of the following statements about DNA is false?
a. DNA contains deoxyribose as its sugar
b. DNA contains the base thymine
c. DNA is double-stranded
d. DNA exists as an alpha-helix
e. None of the above