Ch.20 - BiotechnologySee all chapters
All Chapters
Ch.1 - Introduction to Biology
Ch.2 - Chemistry
Ch.3 - Water
Ch.4 - Carbon
Ch.5 - Biological Molecules
Ch.6 - Cells
Ch.7 - The Membrane
Ch.8 - Energy and Metabolism
Ch.9 - Respiration
Ch.10 - Photosynthesis
Ch.11 - Cell Signaling
Ch.12 - Cell Division
Ch.13 - Meiosis
Ch.14 - Mendelian Genetics
Ch.15 - Chromosomal Theory of Inheritance
Ch.16 - DNA Synthesis
Ch.17 - Gene Expression
Ch.18 - Regulation of Expression
Ch.19 - Viruses
Ch.20 - Biotechnology
Ch.21 - Genomics
Ch.22 - Development
Ch.23 - Evolution by Natural Selection
Ch.24 - Evolution of Populations
Ch.25 - Speciation
Ch.26 - History of Life on Earth
Ch.27 - Phylogeny
Ch.28 - Prokaryotes
Ch.29 - Protists
Ch.30 - Plants
Ch.31 - Fungi
Ch.32 - Overview of Animals
Ch.33 - Invertebrates
Ch.34 - Vertebrates
Ch.35 - Plant Anatomy
Ch.36 - Vascular Plant Transport
Ch.37 - Soil
Ch.38 - Plant Reproduction
Ch.39 - Plant Sensation and Response
Ch.40 - Animal Form and Function
Ch.41 - Digestive System
Ch.42 - Circulatory System
Ch.43 - Immune System
Ch.44 - Osmoregulation and Excretion
Ch.45 - Endocrine System
Ch.46 - Animal Reproduction
Ch.47 - Nervous System
Ch.48 - Sensory Systems
Ch.49 - Muscle Systems
Ch.50 - Ecology
Ch.51 - Animal Behavior
Ch.52 - Population Ecology
Ch.53 - Community Ecology
Ch.54 - Ecosystems
Ch.55 - Conservation Biology

DNA Cloning

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DNA Cloning
PCR and Dideoxy Sequencing
DNA Fingerprinting

Concept #1: Transformation and Restriction Endonucleases

Concept #2: Recombinant Plasmids

Concept #3: Transformation and Antibiotic Resistance

Additional Problems
Restriction enzymes cut only at specific sites and therefore are not useful for genetic engineering.a. Trueb. False
Arrange the following events in the proper sequence for gene cloning.1 = Incorporate gene into bacterial plasmid2 = Isolate DNA from organism containing desired gene3 = Incorporate cloned gene into bacterial cells4 = Fragment DNA with restriction enzymea. 1, 2, 3, 4b. 2, 1, 4, 3 c. 2, 3, 4, 1 d. 2, 4, 1, 3 e. 2, 4, 3, 1 
The function of the enzyme ligase is toa. add new DNA to the plasmid.b. remove DNA from the plasmid.c. form covalent bonds between cloned gene and plasmid.d. repair damaged DNA.e. purify the plasmid DNA.
"Sticky ends" area. single-stranded DNA sequences that are generated by staggered cuts.b. double-stranded DNA sequences that are generated by staggered cuts.c. single-stranded DNA sequences that are generated by blunt cuts.d. double-stranded DNA sequences that are generated by blunt cuts. 
Restriction enzymes cut DNA at random sites.a.Trueb. False
The process used to rapidly produce DNA sequences of interest is DNA sequencing.  a. True b. False
Small accessory rings of DNA are called _____.a. recombinant DNAb. plasmidsc. clonesd. transposons
The plasmid DNA is cleaved by _____.a. DNA ligaseb. DNA polymerasec. a restriction enzymed. helicase 
The foreign DNA is sealed into the opening created by the restriction enzyme by _____.a. DNA polymeraseb. DNA repair enzymesc. DNA ligased. helicase
The single-stranded but complementary ends of the two DNA molecules cleaved by the restriction enzyme _____.a. are called "sticky ends"b. can bind by complementary base pairingc. facilitate the insertion of foreign DNA into vector DNAd.  All of the above
The single-stranded ends of DNA molecules can be joined together bya. restriction endonucleases.b. DNA ligase.c. DNA polymerase.d. primase.e. helicase.
Human DNA cut with restriction enzyme A can be joined toa. human DNA cut with restriction enzyme B.b. human DNA that is uncut.c. bacterial DNA cut with restriction enzyme A.d. bacterial DNA that is uncut.e. none of the above
What is recombinant DNA technology? What are the safety issues related to recombinant DNA technology?
The process of using DNA from one bacterium to alter the characteristics of another is called:A. translationB. transductionC. transcriptionD. transformation
Which ofthe following is a palindromic restriction site?A. 5' - ACGGCA - 3'B. 5' - TGCGCC - 3'C. 5' - AAGGTT - 3'D. 5' - ACGCGT - 3'E. 5' - GCAGCA - 3'
Name the restriction endonuclease that cuts at the following sites. Indicate whether it cuts in a blunt or staggered manner.A. GAATTCB. CTCGAGC. TACGTAD. CTGCAG
During the procedure to form recombinant DNA molecules, the purpose of the cell transformation step is toA. use the transformed cells to combine vector and source DNA in order to form recombinant molecules.B. use the transformed cells as a source of DNA ligase to form recombinant molecules.C. use the transformed cells to cut the DNA at specific sites to generate fragments.D. all the aboveE. None of the above
How is a gene for a particular protein inserted into a plasmid?
What basic structural property of DNA serves as a basis for most techniques used in genetic engineering?A. gene sequencesB. nucleic acid hybridizationC. complete helicesD. antiparallelismE. restriction sites
You would like to clone the DNA of interest into the pDiddy cloning plasmid. You have the following restriction map of the region that includes the DNA of interest and the plasmid (E = EcoRI, H = HindIII, X = XbaI, S = SphI, N = NotI)Which restriction enzyme(s) would you choose to clone the DNA of interest into the cloning vector?A. SB. E and HC. S and ND. X and N
What is Recombinant DNA?
What is a restriction enzyme? What role do they play in biotechnology experiments?
You digest DNA from the frog Xenopus laevis with HindIII that produces sticky DNA ends. You can ligate your DNA to : (check all that apply)A. HindIII sticky DNA ends from Xenopus laevisB. EcoRI sticky DNA ends from Xenopus laevisC. blunt DNA ends from bacteriaD. HindIII sticky DNA ends from bacteria
Jason is setting up a reaction with the restriction endonuclease EcoRI. The concentration of the EcoRI enzyme solution is 20,000 units/mL. Jason mixes the following in a test tube:4 uL dsDNA, 14 uL buffer, 2 uL EcoRIWhat is the final concentration of EcoRI enzyme in Jason's reaction?A. 2,222 units/mLB. 2,857 units/mLC. 2,000 units/mLD. 200,000 units/mL