Clutch Prep is now a part of Pearson
Ch. 6 - Enzymes and Enzyme KineticsWorksheetSee all chapters
All Chapters
Ch. 1 - Introduction to Biochemistry
Ch. 2 - Water
Ch. 3 - Amino Acids
Ch. 4 - Protein Structure
Ch. 5 - Protein Techniques
Ch. 6 - Enzymes and Enzyme Kinetics
Ch. 7 - Enzyme Inhibition and Regulation
Ch. 8 - Protein Function
Ch. 9 - Carbohydrates
Ch. 10 - Lipids
Ch. 11 - Biological Membranes and Transport
Ch. 12 - Biosignaling
Clutch Review 1: Nucleic Acids, Lipids, & Membranes
Clutch Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway
Clutch Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism
Clutch Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation
Sections
Enzymes
Enzyme-Substrate Complex
Lock and Key Vs. Induced Fit Models
Optimal Enzyme Conditions
Activation Energy
Types of Enzymes
Cofactor
Catalysis
Electrostatic and Metal Ion Catalysis
Covalent Catalysis
Reaction Rate
Enzyme Kinetics
Rate Constants and Rate Law
Reaction Orders
Rate Constant Units
Initial Velocity
Vmax Enzyme
Km Enzyme
Steady-State Conditions
Michaelis-Menten Assumptions
Michaelis-Menten Equation
Lineweaver-Burk Plot
Michaelis-Menten vs. Lineweaver-Burk Plots
Shifting Lineweaver-Burk Plots
Calculating Vmax
Calculating Km
Kcat
Specificity Constant

Concept #1: Introduction to the Six Major Classes of Enzymes 

Concept #2: Oxidoreductases

Practice: Which of the following is an indicator that a reaction is catalyzed by an oxidoreductase?

Concept #3: Transferases

Practice: Kinases add phosphate groups to molecules. Kinases are an example of which class of enzyme?

Concept #4: Hydrolyases

Practice: Below is a reaction in the oxidative phase of the pentose phosphate pathway catalyzed by the enzyme gluconolactonase. This enzyme would be classified as what type?


Concept #5: Isomerases

Practice: What class of enzyme is required to convert a cis-fatty acid into a trans-fatty acid?

Concept #6: Lyases

Practice: Which of the following best describes the function of a lyase enzyme?

Concept #7: Ligases

Practice: Which of the following is not one of the six internationally accepted classes of enzymes?

a) Hydrolyases.

b) Ligases.

c) Oxidoreductases.

d) Polymerases.

e) Transferases.

Concept #8: Recap of the Six Classes of Enzymes 

Practice: The presence of an electron acceptor such as NAD + indicates which class of enzyme?

Practice: Digestive enzymes are all examples of which class of enzyme?

Practice: The reaction below is catalyzed by the enzyme arginase. What is the classification of this enzyme?

a) Hydrolyase. 

b) Oxidoreductase. 

c) Lyase. 

d) Ligase.

Practice: Which class of enzyme catalyzes a reaction where the chemical formula does not change?

Practice: What class of enzyme is required to combine two molecules using ATP?