Concept #1: Titration Curve Review
Concept #2: Titrations Of Amino Acids With Non-Ionizable R-Groups
Practice: Draw the predominate structures of Leu at each of the indicated sections ( 1, 2, 3) on its titration curve.
Practice: Calculate the pI of Ile using its titration curve. Mark the approximate position of the pI on the titration curve.
Practice: Which of the following compounds would make for the best buffer at pH 8?
Practice: Identify the region(s) on glycine’s titration (I, II, III, IV, or V) that corresponds with each statement below:
a. Region where Gly predominant species has net charge of +1. ________
b. Region where the average net charge of Gly is +½. ________
c. Region where ½ of Gly’s amino groups are ionized. ________
d. Region where the pH = pKa of carboxyl group. ________
e. Region where the pH = pKa of amino group. ________
f. Regions where Gly has its maximum buffering capacity. _____________
g. Region where the average net charge of Gly is 0. ________
h. Region where Gly’s carboxyl group has been completely titrated. ________
i. Region where Gly has been completely titrated. ________
j. Region where Gly’s predominant species is a zwitterion. ________
k. Region where the average net charge of Gly is -1. ________
l. Region where Gly is a 50:50 mixture of protonated & deprotonated carboxyl group. ________
m. Region indicating Gly’s isoelectric point (pI). ________
n. Region indicating the end of Gly’s titration. ________
o. Regions where Gly has poor buffering power. ____________________