Concept #1: Titrations Of Amino Acids With Ionizable R-Groups

Concept #2: Analyzing Titrations Of Amino Acids With Ionizable R-Groups

Practice: Determine the pI of Glu & mark it on the provided titration curve.

a. 6.96                                          

b. 5.93

c. 7.48

d. 3.22

Use the titration curve to draw the predominate structures of Glu at pH = 1.0, pH = 3.0, pH = 7.0, & pH = 11.0.

Glu at pH = 1.0                  Glu at pH = 3.0                  Glu at pH = 7.0                   Glu at pH = 11.0

Practice: Draw the predominate structures of K at the indicated sections of its titration curve. Mark the pI on the curve.

Practice: Label each arrow on Asp’s titration curve & draw its predominate structure at each colored region.

A) Amino group pKa.

B) Carboxyl group pKa.

C) R-group pKa (pKa = 3.9).

D) Amino group equivalence point.

E) Carboxyl group equivalence point.

F) R-group equivalence point.