Biochemistry Biochemistry

Biochemistry Garrett & Grisham • 6th Edition • 978-1305577206

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Ch. 1 - The Facts of Life: Chemistry Is the Logic of Biological Phenomena

What is Biochemistry? Characteristics of Life Abiogenesis Nucleic Acids Proteins Carbohydrates Lipids Taxonomy Cell Organelles Endosymbiotic Theory Central Dogma Functional Groups Chemical Bonds Organic Chemistry

Ch. 2 - Water: The Medium of Life

Properties of Water Osmosis Hydrophobic Effect Acids and Bases Autoionization of Water pH Acid Dissociation Constant Henderson Hasselbalch Equation Determining Predominate Species Titration Buffer Solution

Ch. 3 - Thermodynamics of Biological Systems

Entropy Second Law of Thermodynamics Equilibrium Constant Gibbs Free Energy

Ch. 4, 5, & 6 - Amino Acids, Proteins, & Techniques

Amino Acid Groups Amino Acid Three Letter Code Amino Acid One Letter Code Amino Acid Configuration Essential Amino Acids Nonpolar Amino Acids Aromatic Amino Acids Polar Amino Acids Charged Amino Acids How to Memorize Amino Acids Zwitterion Non-Ionizable Vs. Ionizable R-Groups Isoelectric Point Isoelectric Point of Amino Acids with Ionizable R-Groups Titrations of Amino Acids with Non-Ionizable R-Groups Titrations of Amino Acids with Ionizable R-Groups Amino Acids and Henderson-Hasselbalch Peptide Bond Primary Structure of Protein Altering Primary Protein Structure Drawing a Peptide Determining Net Charge of a Peptide Isoelectric Point of a Peptide Approximating Protein Mass Peptide Group Ramachandran Plot Atypical Ramachandran Plots Alpha Helix Alpha Helix Pitch and Rise Alpha Helix Hydrogen Bonding Alpha Helix Disruption Beta Strand Beta Sheet Antiparallel and Parallel Beta Sheets Beta Turns Tertiary Structure of Protein Protein Motifs and Domains Denaturation Anfinsen Experiment Protein Folding Chaperone Proteins Quaternary Structure Simple Vs. Conjugated Proteins Fibrous and Globular Proteins Protein Purification Protein Extraction Differential Centrifugation Salting Out Dialysis Column Chromatography Ion-Exchange Chromatography Anion-Exchange Chromatography Size Exclusion Chromatography Affinity Chromatography Specific Activity HPLC Spectrophotometry Native Gel Electrophoresis SDS-PAGE SDS-PAGE Strategies Isoelectric Focusing 2D-Electrophoresis Diagonal Electrophoresis Mass Spectrometry Mass Spectrum Tandem Mass Spectrometry Peptide Mass Fingerprinting Overview of Direct Protein Sequencing Amino Acid Hydrolysis FDNB Chemical Cleavage of Bonds Peptidases Edman Degradation Edman Degradation Sequenator and Sequencing Data Analysis Edman Degradation Reaction Efficiency Ordering Cleaved Fragments Strategy for Ordering Cleaved Fragments Indirect Protein Sequencing Via Geneomic Analyses

Clutch Review 1: Enzymes: Binding, Kinetics & Inhibition

Binding Affinity Binding Affinity Practice O2 Transportation Enzymes Michaelis-Menten Model Lineweaver-Burk Model Enzyme Kinetics Practice Competitive and Uncompetitive Inhibition Mixed Inhibition Inhibition Practice

Clutch Review 2: Nucleic Acids, Lipids, & Membranes

Nucleic Acids 1 Nucleic Acids 2 Nucleic Acids 3 Nucleic Acids 4 DNA Sequencing 1 DNA Sequencing 2 Lipids 1 Lipids 2 Membrane Structure 1 Membrane Structure 2 Membrane Transport 1 Membrane Transport 2 Membrane Transport 3 Nucleic Acids 1 Nucleic Acids 2 Nucleic Acids 3 Lipids Membrane Structure 1 Membrane Structure 2 Membrane Transport 1 Membrane Transport 2

Clutch Review 3: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway

Biosignaling 1 Biosignaling 2 Biosignaling 3 Biosignaling 4 Glycolysis 1 Glycolysis 2 Glycolysis 3 Glycolysis 4 Fermentation Gluconeogenesis 1 Gluconeogenesis 2 Pentose Phosphate Pathway Practice - Biosignaling Practice - Bioenergetics 1 Practice - Bioenergetics 2 Practice - Glycolysis 1 Practice - Glycolysis 2 Practice - Gluconeogenesis Practice - Pentose Phosphate Path

Clutch Review 4: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism

Pyruvate Oxidation Citric Acid Cycle 1 Citric Acid Cycle 2 Citric Acid Cycle 3 Citric Acid Cycle 4 Metabolic Regulation 1 Metabolic Regulation 2 Glycogen Metabolism 1 Glycogen Metabolism 2 Fatty Acid Oxidation 1 Fatty Acid Oxidation 2 Citric Acid Cycle Practice 1 Citric Acid Cycle Practice 2 Citric Acid Cycle Practice 3 Glucose and Glycogen Regulation Practice 1 Glucose and Glycogen Regulation Practice 2 Fatty Acid Oxidation Practice 1 Fatty Acid Oxidation Practice 2

Clutch Review 5: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation

Amino Acid Oxidation 1 Amino Acid Oxidation 2 Oxidative Phosphorylation 1 Oxidative Phosphorylation 2 Oxidative Phosphorylation 3 Oxidative Phosphorylation 4 Photophosphorylation 1 Photophosphorylation 2 Photophosphorylation 3 Practice: Amino Acid Oxidation 1 Practice: Amino Acid Oxidation 2 Practice: Oxidative Phosphorylation 1 Practice: Oxidative Phosphorylation 2 Practice: Oxidative Phosphorylation 3 Practice: Photophosphorylation 1 Practice: Photophosphorylation 2

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