Practice: Use the plot below. The Km of both enzymes for their substrate is:
Concept #1: Shifting Lineweaver-Burk Plots
Concept #2: Visualizing Increases/Decreases to Km & Vmax
Example #1: Indicate which enzyme in each graph below has a greater Km & Vmax.
Practice: Suppose a company develops two enzymes that degrade highly toxic compounds to non-toxic compounds. Your task is to degrade the greatest amount of toxic compound in the shortest amount of time.
A) Which enzyme is better to use when [S] = 0.167 mM? ____________
B) Which enzyme is better to use at saturating [S]? ____________
Practice: Considering the Lineweaver-Burk plot below, which of the following enzymes would be better to use (converts more substrate to product) when the [S] = 0.001 M?
