Practice: Compare the Native & SDS PAGE gels to indicate if each sample is a monomer, dimer, trimer or tetramer.
a. Sample 1: ________________
b. Sample 2: ________________
c. Sample 3: ________________
d. Sample 4: ________________
Subjects
Sections | |||
---|---|---|---|
Protein Purification | 8 mins | 0 completed | Learn |
Protein Extraction | 6 mins | 0 completed | Learn |
Differential Centrifugation | 15 mins | 0 completed | Learn |
Salting Out | 19 mins | 0 completed | Learn |
Dialysis | 10 mins | 0 completed | Learn |
Column Chromatography | 11 mins | 0 completed | Learn |
Ion-Exchange Chromatography | 35 mins | 0 completed | Learn |
Anion-Exchange Chromatography | 38 mins | 0 completed | Learn |
Size Exclusion Chromatography | 31 mins | 0 completed | Learn |
Affinity Chromatography | 17 mins | 0 completed | Learn |
Specific Activity | 17 mins | 0 completed | Learn |
HPLC | 30 mins | 0 completed | Learn |
Spectrophotometry | 51 mins | 0 completed | Learn |
Native Gel Electrophoresis | 23 mins | 0 completed | Learn |
SDS-PAGE | 34 mins | 0 completed | Learn |
SDS-PAGE Strategies | 17 mins | 0 completed | Learn |
Isoelectric Focusing | 20 mins | 0 completed | Learn |
2D-Electrophoresis | 27 mins | 0 completed | Learn |
Diagonal Electrophoresis | 29 mins | 0 completed | Learn |
Mass Spectrometry | 13 mins | 0 completed | Learn |
Mass Spectrum | 48 mins | 0 completed | Learn |
Tandem Mass Spectrometry | 17 mins | 0 completed | Learn |
Peptide Mass Fingerprinting | 17 mins | 0 completed | Learn |
Overview of Direct Protein Sequencing | 30 mins | 0 completed | Learn |
Amino Acid Hydrolysis | 11 mins | 0 completed | Learn |
FDNB | 30 mins | 0 completed | Learn |
Chemical Cleavage of Bonds | 33 mins | 0 completed | Learn |
Peptidases | 66 mins | 0 completed | Learn |
Edman Degradation | 38 mins | 0 completed | Learn |
Edman Degradation Sequenator and Sequencing Data Analysis | 9 mins | 0 completed | Learn |
Edman Degradation Reaction Efficiency | 21 mins | 0 completed | Learn |
Ordering Cleaved Fragments | 22 mins | 0 completed | Learn |
Strategy for Ordering Cleaved Fragments | 59 mins | 0 completed | Learn |
Indirect Protein Sequencing Via Geneomic Analyses | 24 mins | 0 completed | Learn |
Practice: Compare the Native & SDS PAGE gels to indicate if each sample is a monomer, dimer, trimer or tetramer.
a. Sample 1: ________________
b. Sample 2: ________________
c. Sample 3: ________________
d. Sample 4: ________________
Practice: “Protein X” has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When subjected to SDS-PAGE, Protein X gives 3 bands with molecular masses of 180, 160, & 60 kDa. When SDS-PAGE is conducted a second time but in the presence of β-mercaptoethanol (β-ME), 3 bands form again, but this time with molecular masses of 160, 90, and 60 kDa. What is the subunit composition of Protein X? (Hint: draw both SDS-PAGE gels).
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