Ch. 7 - Enzyme Inhibition and Regulation WorksheetSee all chapters
All Chapters
Ch. 1 - Introduction to Biochemistry
Ch. 2 - Water
Ch. 3 - Amino Acids
Ch. 4 - Protein Structure
Ch. 5 - Protein Techniques
Ch. 6 - Enzymes and Enzyme Kinetics
Ch. 7 - Enzyme Inhibition and Regulation
Ch. 8 - Protein Function
Ch. 9 - Carbohydrates
Ch. 10 - Lipids
Ch. 11 - Biological Membranes and Transport
Ch. 12 - Biosignaling
Clutch Review 1: Nucleic Acids, Lipids, & Membranes
Clutch Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway
Clutch Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism
Clutch Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation
Enzyme Inhibition
Irreversible Inhibition
Reversible Inhibition
Inhibition Constant
Degree of Inhibition
Apparent Km and Vmax
Inhibition Effects on Reaction Rate
Competitive Inhibition
Uncompetitive Inhibition
Mixed Inhibition
Noncompetitive Inhibition
Recap of Reversible Inhibition
Allosteric Regulation
Allosteric Kinetics
Allosteric Enzyme Conformations
Allosteric Effectors
Concerted (MWC) Model
Sequential (KNF) Model
Negative Feedback
Positive Feedback
Post Translational Modification

Concept #1: Post Translational Modification

Concept #2: Proteolytic Cleavage

Practice: Which of the following would not be a reversible post-translational modification?

Practice: An enzyme’s active site has high affinity for a polar charged substrate. How will methylation of the active site affect the reaction rate of the enzyme?

Practice: Histones are proteins that regulate gene expression by binding to DNA and controlling which regions are exposed to be expressed. Histones bind to DNA at a neutral pH via their positively charged lysine residues, but acetylation of histones leads to the dissociation of the DNA-Histone complex. Covalent modification of lysine’s ammonium group by acetylation results in an overall _______________ charge of the histone binding region.