Concept #1: Phosphorylation

Concept #2: ATP is a Common Source of Phosphorylation

Practice: During kinase phosphorylation, which phosphate group is removed from ATP in the figure below?

Concept #3: Amino Acid Phosphorylation

Practice: Covalent modification of an enzyme usually involves phosphorylation / dephosphorylation of:

Practice: When the active site of an enzyme is phosphorylated on one of its catalytic amino acid residues, the overall _______________ charge of phosphate groups would _____________ the affinity for a polar, negatively charged substrate.