Ch. 5 - Protein TechniquesWorksheetSee all chapters
All Chapters
Ch. 1 - Introduction to Biochemistry
Ch. 2 - Water
Ch. 3 - Amino Acids
Ch. 4 - Protein Structure
Ch. 5 - Protein Techniques
Ch. 6 - Enzymes and Enzyme Kinetics
Ch. 7 - Enzyme Inhibition and Regulation
Ch. 8 - Protein Function
Ch. 9 - Carbohydrates
Ch. 10 - Lipids
Ch. 11 - Biological Membranes and Transport
Ch. 12 - Biosignaling
Clutch Review 1: Nucleic Acids, Lipids, & Membranes
Clutch Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway
Clutch Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism
Clutch Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation
Protein Purification
Protein Extraction
Differential Centrifugation
Salting Out
Column Chromatography
Ion-Exchange Chromatography
Anion-Exchange Chromatography
Size Exclusion Chromatography
Affinity Chromatography
Specific Activity
Native Gel Electrophoresis
SDS-PAGE Strategies
Isoelectric Focusing
Diagonal Electrophoresis
Mass Spectrometry
Mass Spectrum
Tandem Mass Spectrometry
Peptide Mass Fingerprinting
Overview of Direct Protein Sequencing
Amino Acid Hydrolysis
Chemical Cleavage of Bonds
Edman Degradation
Edman Degradation Sequenator and Sequencing Data Analysis
Edman Degradation Reaction Efficiency
Ordering Cleaved Fragments
Strategy for Ordering Cleaved Fragments
Indirect Protein Sequencing Via Geneomic Analyses

Concept #1: Overview Of Direct Protein Sequencing

Practice: Which of the following is a protein sequencing technique?

Concept #2: Overview of Techniques for Protein Sequencing

Practice: Appropriately match each option to each description. Options may be used more than once.

a. Cyanogen bromide (CNBr).              _____ An enzyme that cleaves specific peptide bonds.

b. Edman Degradation.                        _____ Nonspecifically cleaves all peptide bonds.          

c. Elastase.                                           _____ A chemical that breaks specific peptide bonds.

d. 6M HCl.                                             _____ Used as an N-terminal protein sequencing technique.

                                                              _____ Used as a peptide cleavage technique.

                                                              _____ Results in smaller peptide fragments.

                                                              _____ Results in free amino acids.

Practice: Appropriately match each option to each reagent. Options may be used more than once.

a. Amino acid hydrolysis.                           _____ Hydrazine.

b. Chemical cleavage.                                _____ Trypsin.           

c. Peptidase.                                              _____ 6M Hydrochloric acid.

                                                                   ______ β-mercaptoethanol + iodoacetate.

                                                                   ______ Chymotrypsin.