Practice: Which advantage does native gel electrophoresis provide as a protein technique?
Subjects
Sections | |||
---|---|---|---|
Protein Purification | 8 mins | 0 completed | Learn |
Protein Extraction | 6 mins | 0 completed | Learn |
Differential Centrifugation | 15 mins | 0 completed | Learn |
Salting Out | 19 mins | 0 completed | Learn |
Dialysis | 10 mins | 0 completed | Learn |
Column Chromatography | 11 mins | 0 completed | Learn |
Ion-Exchange Chromatography | 35 mins | 0 completed | Learn |
Anion-Exchange Chromatography | 38 mins | 0 completed | Learn |
Size Exclusion Chromatography | 31 mins | 0 completed | Learn |
Affinity Chromatography | 17 mins | 0 completed | Learn |
Specific Activity | 17 mins | 0 completed | Learn |
HPLC | 30 mins | 0 completed | Learn |
Spectrophotometry | 51 mins | 0 completed | Learn |
Native Gel Electrophoresis | 23 mins | 0 completed | Learn |
SDS-PAGE | 34 mins | 0 completed | Learn |
SDS-PAGE Strategies | 17 mins | 0 completed | Learn |
Isoelectric Focusing | 20 mins | 0 completed | Learn |
2D-Electrophoresis | 27 mins | 0 completed | Learn |
Diagonal Electrophoresis | 29 mins | 0 completed | Learn |
Mass Spectrometry | 13 mins | 0 completed | Learn |
Mass Spectrum | 48 mins | 0 completed | Learn |
Tandem Mass Spectrometry | 17 mins | 0 completed | Learn |
Peptide Mass Fingerprinting | 17 mins | 0 completed | Learn |
Overview of Direct Protein Sequencing | 30 mins | 0 completed | Learn |
Amino Acid Hydrolysis | 11 mins | 0 completed | Learn |
FDNB | 30 mins | 0 completed | Learn |
Chemical Cleavage of Bonds | 33 mins | 0 completed | Learn |
Peptidases | 66 mins | 0 completed | Learn |
Edman Degradation | 38 mins | 0 completed | Learn |
Edman Degradation Sequenator and Sequencing Data Analysis | 9 mins | 0 completed | Learn |
Edman Degradation Reaction Efficiency | 21 mins | 0 completed | Learn |
Ordering Cleaved Fragments | 22 mins | 0 completed | Learn |
Strategy for Ordering Cleaved Fragments | 59 mins | 0 completed | Learn |
Indirect Protein Sequencing Via Geneomic Analyses | 24 mins | 0 completed | Learn |
Concept #1: Native-PAGE
Practice: Which advantage does native gel electrophoresis provide as a protein technique?
Practice: Which option below best describes the native gel electrophoresis migration for Proteins A, B, C & D (assuming equal mass & shape) considering that the buffer solution has a pH = 6.4.
Protein A pI = 5.2, Protein B pI = 6.4, Protein C pI = 7.0, Protein D pI = 9.2
Practice: A) Consider both the peptide Gly—Pro—Ser—Glu—Thr (in a linear chain) and a cyclic peptide of the same exact sequence Gly—Pro—Ser—Glu—Thr (with a peptide bond linking the Thr & Gly). Are these peptides chemically the same? Explain.
B) Can you expect to separate the peptides above by Native-PAGE? Why or why not?
Join thousands of students and gain free access to 14 hours of Biochemistry videos that follow the topics your textbook covers.
Enter your friends' email addresses to invite them: