Concept #1: Lineweaver-Burk Equation
Concept #2: Lineweaver-Burk Plot
Practice: A Lineweaver-Burk plot is used to:
Concept #3: X & Y-Intercepts of a Lineweaver-Burk Plot
Practice: To determine the Vmax from a Lineweaver-Burk plot you would:
Practice: A Lineweaver-Burk plot generates a line with the following formula: y = 0.3x + 0.4. What is the Km?
Practice: Consider the equation for the line on the following Lineweaver-Burk plot: y = 6x + 3
A) What is the Km for the corresponding enzyme?
a) -2 mM
b) 18 mM
c) 2 mM
d) 9 mM
B) What is the Vmax for the corresponding enzyme?
Practice: Suppose the data on the right is plotted onto a Lineweaver-Burk plot to obtain a straight line.
A) What is the value of the y-intercept of the line? ____________
B) What is the value of the x-intercept of the line? ____________