Clutch Prep is now a part of Pearson
Ch. 6 - Enzymes and Enzyme KineticsWorksheetSee all chapters
All Chapters
Ch. 1 - Introduction to Biochemistry
Ch. 2 - Water
Ch. 3 - Amino Acids
Ch. 4 - Protein Structure
Ch. 5 - Protein Techniques
Ch. 6 - Enzymes and Enzyme Kinetics
Ch. 7 - Enzyme Inhibition and Regulation
Ch. 8 - Protein Function
Ch. 9 - Carbohydrates
Ch. 10 - Lipids
Ch. 11 - Biological Membranes and Transport
Ch. 12 - Biosignaling
Clutch Review 1: Nucleic Acids, Lipids, & Membranes
Clutch Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway
Clutch Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism
Clutch Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation
Sections
Enzymes
Enzyme-Substrate Complex
Lock and Key Vs. Induced Fit Models
Optimal Enzyme Conditions
Activation Energy
Types of Enzymes
Cofactor
Catalysis
Electrostatic and Metal Ion Catalysis
Covalent Catalysis
Reaction Rate
Enzyme Kinetics
Rate Constants and Rate Law
Reaction Orders
Rate Constant Units
Initial Velocity
Vmax Enzyme
Km Enzyme
Steady-State Conditions
Michaelis-Menten Assumptions
Michaelis-Menten Equation
Lineweaver-Burk Plot
Michaelis-Menten vs. Lineweaver-Burk Plots
Shifting Lineweaver-Burk Plots
Calculating Vmax
Calculating Km
Kcat
Specificity Constant

Concept #1: Lineweaver-Burk Equation

Concept #2: Lineweaver-Burk Plot

Practice: A Lineweaver-Burk plot is used to:

Concept #3: X & Y-Intercepts of a Lineweaver-Burk Plot

Practice: To determine the Vmax from a Lineweaver-Burk plot you would:

Practice: A Lineweaver-Burk plot generates a line with the following formula: y = 0.3x + 0.4. What is the Km?

Practice: Consider the equation for the line on the following Lineweaver-Burk plot: y = 6x + 3

A) What is the Km for the corresponding enzyme?

a) -2 mM 

b) 18 mM 

c) 2 mM 

d) 9 mM

B) What is the Vmax for the corresponding enzyme?

a) 0.333 mM/s 

b) 9 mM/s 

c) -6 mM/s 

d) 18 mM/s

Practice: Suppose the data on the right is plotted onto a Lineweaver-Burk plot to obtain a straight line.


A) What is the value of the y-intercept of the line? ____________

B) What is the value of the x-intercept of the line? ____________