Concept #1: Km Enzyme
Practice: What is the initial velocity of a reaction when the concentration of substrate is set equal to the Km?
Concept #2: Km Can Be Expressed with Rate Constants
Practice: Select the best description of the K m.
Concept #3: Km Measures an Enzyme’s Affinity for its Substrate
Practice: According to the chart below, which one of the following enzymes has the strongest affinity for its substrate?
Concept #4: [E]T Does Not Affect the Km
Practice: Indicate which region of the Enzyme Kinetics plot below best corresponds to each statement.
A) Initial reaction velocity is limited mainly by the [S] present: ______
B) Initial reaction velocity limited mainly by the [E] present: ______
C) The active site of an enzyme is most likely free/unoccupied: ______
D) The active site of an enzyme is most likely occupied by substrate: ______
E) This region includes the points corresponding to Km & ½Vmax: ______
Practice: Use the data in the following chart to determine the Km of the enzyme.