Practice: At some point during isoelectric focusing, proteins stop moving through the gel because:
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|---|---|---|---|
| Protein Purification | 8 mins | 0 completed | Learn |
| Protein Extraction | 6 mins | 0 completed | Learn |
| Differential Centrifugation | 15 mins | 0 completed | Learn |
| Salting Out | 19 mins | 0 completed | Learn |
| Dialysis | 10 mins | 0 completed | Learn |
| Column Chromatography | 11 mins | 0 completed | Learn |
| Ion-Exchange Chromatography | 35 mins | 0 completed | Learn |
| Anion-Exchange Chromatography | 38 mins | 0 completed | Learn |
| Size Exclusion Chromatography | 29 mins | 0 completed | Learn |
| Affinity Chromatography | 17 mins | 0 completed | Learn |
| Specific Activity | 17 mins | 0 completed | Learn |
| HPLC | 30 mins | 0 completed | Learn |
| Spectrophotometry | 51 mins | 0 completed | Learn |
| Native Gel Electrophoresis | 23 mins | 0 completed | Learn |
| SDS-PAGE | 34 mins | 0 completed | Learn |
| SDS-PAGE Strategies | 17 mins | 0 completed | Learn |
| Isoelectric Focusing | 18 mins | 0 completed | Learn |
| 2D-Electrophoresis | 27 mins | 0 completed | Learn |
| Diagonal Electrophoresis | 29 mins | 0 completed | Learn |
| Mass Spectrometry | 13 mins | 0 completed | Learn |
| Mass Spectrum | 48 mins | 0 completed | Learn |
| Tandem Mass Spectrometry | 17 mins | 0 completed | Learn |
| Peptide Mass Fingerprinting | 17 mins | 0 completed | Learn |
| Overview of Direct Protein Sequencing | 30 mins | 0 completed | Learn |
| Amino Acid Hydrolysis | 11 mins | 0 completed | Learn |
| FDNB | 27 mins | 0 completed | Learn |
| Chemical Cleavage of Bonds | 33 mins | 0 completed | Learn |
| Peptidases | 66 mins | 0 completed | Learn |
| Edman Degradation | 38 mins | 0 completed | Learn |
| Edman Degradation Sequenator and Sequencing Data Analysis | 9 mins | 0 completed | Learn |
| Edman Degradation Reaction Efficiency | 21 mins | 0 completed | Learn |
| Ordering Cleaved Fragments | 22 mins | 0 completed | Learn |
| Strategy for Ordering Cleaved Fragments | 59 mins | 0 completed | Learn |
| Indirect Protein Sequencing Via Geneomic Analyses | 24 mins | 0 completed | Learn |
Concept #1: Isoelectric Focusing
Practice: Mark the approximate final position of the following tripeptide on the isoelectric focusing gel: Glu-Met-Asp.
Hint: calculate the isoelectric point of the peptide.
Progress
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Videos in Isoelectric Focusing
Concept #1: Isoelectric Focusing
Practice #1: Isoelectric Focusing Practice 1
Practice #2: Isoelectric Focusing Practice 3
