Ch. 12 - BiosignalingWorksheetSee all chapters
All Chapters
Ch. 1 - Introduction to Biochemistry
Ch. 2 - Water
Ch. 3 - Amino Acids
Ch. 4 - Protein Structure
Ch. 5 - Protein Techniques
Ch. 6 - Enzymes and Enzyme Kinetics
Ch. 7 - Enzyme Inhibition and Regulation
Ch. 8 - Protein Function
Ch. 9 - Carbohydrates
Ch. 10 - Lipids
Ch. 11 - Biological Membranes and Transport
Ch. 12 - Biosignaling
Clutch Review 1: Nucleic Acids, Lipids, & Membranes
Clutch Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway
Clutch Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism
Clutch Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation
Sections
Introduction to Biosignaling
G protein-Coupled Receptors
Stimulatory Adenylate Cyclase GPCR Signaling
cAMP & PKA
Inhibitory Adenylate Cyclase GPCR Signaling
Drugs & Toxins Affecting GPCR Signaling
Recap of Adenylate Cyclase GPCR Signaling
Phosphoinositide GPCR Signaling
PSP Secondary Messengers & PKC
Recap of Phosphoinositide Signaling
Receptor Tyrosine Kinases
Insulin
Insulin Receptor
Insulin Signaling on Glucose Metabolism
Recap Of Insulin Signaling in Glucose Metabolism
Insulin Signaling as a Growth Factor
Recap of Insulin Signaling As A Growth Factor
Recap of Insulin Signaling
Jak-Stat Signaling
Lipid Hormone Signaling
Summary of Biosignaling
Signaling Defects & Cancer

Concept #1: Insulin Signaling Decreases Blood [Glucose]

Example #1: If you delete the PKB gene from insulin-responsive cells, what would you likely observe in insulin’s presence?

Concept #2: Insulin Signaling Activates Glycogen Synthesis

Practice: Place the following insulin signaling transduction events in order of occurrence (event 9 is provided).

a) Full activation of PKB/Akt ______.

b) Activated PI3K converts PIP2 to PIP3 ______.

c) GLUT4 expressed in the cell membrane ___9___.

d) Ligand/insulin binding to the insulin receptor ______.

e) GSK3 is phosphorylated & inactivated ______.

f) Activated PDK1 phosphorylates & activates of PKB/Akt ______.

g) IRS-1 is phosphorylated & activated by the insulin receptor ______.

h) Autophosphorylation of the insulin receptor ______.

i) PI3K is activated upon its SH2 domain binding phosphorylated IRS-1 ______.

j) Glycogen synthase converts glucose to glycogen ______.

k) PIP3 molecules laterally diffuse to bind PKB/Akt & PDK1 ______.

Concept #3: How to Remember Insulin RTK Signaling on Glucose Metabolism?

Example #2: Place the following insulin signal transduction events in order of occurrence (1-8).

Practice: All of the following are true of the reaction catalyzed by PI3K EXCEPT:

Practice: Which of the following results would you predict to occur if a mutation changes a Tyr residue of the Insulin Receptor to Ala?

Practice: IRS-1 is an essential adaptor protein in the insulin signaling pathway. If IRS-1 was under expressed in muscle cells, what effect would you expect to see on glycogen synthesis?