Ch. 12 - BiosignalingWorksheetSee all chapters
All Chapters
Ch. 1 - Introduction to Biochemistry
Ch. 2 - Water
Ch. 3 - Amino Acids
Ch. 4 - Protein Structure
Ch. 5 - Protein Techniques
Ch. 6 - Enzymes and Enzyme Kinetics
Ch. 7 - Enzyme Inhibition and Regulation
Ch. 8 - Protein Function
Ch. 9 - Carbohydrates
Ch. 10 - Lipids
Ch. 11 - Biological Membranes and Transport
Ch. 12 - Biosignaling
Clutch Review 1: Nucleic Acids, Lipids, & Membranes
Clutch Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway
Clutch Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism
Clutch Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation
Sections
Introduction to Biosignaling
G protein-Coupled Receptors
Stimulatory Adenylate Cyclase GPCR Signaling
cAMP & PKA
Inhibitory Adenylate Cyclase GPCR Signaling
Drugs & Toxins Affecting GPCR Signaling
Recap of Adenylate Cyclase GPCR Signaling
Phosphoinositide GPCR Signaling
PSP Secondary Messengers & PKC
Recap of Phosphoinositide Signaling
Receptor Tyrosine Kinases
Insulin
Insulin Receptor
Insulin Signaling on Glucose Metabolism
Recap Of Insulin Signaling in Glucose Metabolism
Insulin Signaling as a Growth Factor
Recap of Insulin Signaling As A Growth Factor
Recap of Insulin Signaling
Jak-Stat Signaling
Lipid Hormone Signaling
Summary of Biosignaling
Signaling Defects & Cancer

Concept #1: Map of Lesson on Biosignaling Pathways

Concept #2: Insulin Growth Factor Signaling via Ras Pathway

Example #1: G proteins such as Ras are activated when:

Practice: In the insulin signaling pathway to activate the protein Ras, activated IRS-1 binds to ___________.

Concept #4: Ras Pathway Activates the MAPK Signaling Pathway

Example #2: All of the following are true in the Ras and MAPK pathways EXCEPT:

Concept #5: Termination of Insulin Signaling as a Growth Factor

Practice: The monomeric G-protein Ras is activated by _______________, while _______________ helps turn Ras off.

Concept #6: How to Remember Insulin RTK Signaling as a Growth Factor?

Practice: Place the insulin RTK signaling events in order from 1-7:

a) Ligand binding results in autophosphorylation of the receptor tyrosine kinase ______.

b) MAPKK (Mek) phosphorylates MAPK (Erk) ______.

c) Sos binds then activates Ras ______.

d) Receptor tyrosine kinase phosphorylate IRS-1, which binds the SH2 domain of Grb2 ______.

e) Ras activates MAPKKK (Raf-1) ______.

f) MAPK (Erk) activates transcription factors to regulate mRNA synthesis ______.

g) MAPKKK (Raf-1) phosphorylates MAPKK (Mek) _______.

Practice: Ras is estimated to be responsible for 30% of human cancers. Most mutations in Ras prevent the protein from binding GTPase-activation proteins (GAPs). What is the effect of this mutation on the signal transduction?

Practice: Insulin is one of the hormones/growth factors that will activate the Ras and MAP kinase pathway. Concerning this system, all of the following are true EXCEPT

Practice: All of the following are true in the Ras and MAPK pathway EXCEPT:

Practice: Guanine Exchange Factors (GEFs) bind Ras-GDP and promote dissociation of bound GDP to be replaced by GTP. GTPase Activating Proteins (GAPs) bind Ras-GTP and stimulates GTPase activity of Ras. How is the downstream signal affected by the presence of GEFs and GAPs?