Practice: What is the order of elution (first → last) of the following amino acids in normal-phase HPLC: Phe, Gly, Glu.
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| Protein Purification | 8 mins | 0 completed | Learn |
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| HPLC | 30 mins | 0 completed | Learn |
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| Peptide Mass Fingerprinting | 17 mins | 0 completed | Learn |
| Overview of Direct Protein Sequencing | 30 mins | 0 completed | Learn |
| Amino Acid Hydrolysis | 11 mins | 0 completed | Learn |
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| Chemical Cleavage of Bonds | 33 mins | 0 completed | Learn |
| Peptidases | 66 mins | 0 completed | Learn |
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| Edman Degradation Sequenator and Sequencing Data Analysis | 9 mins | 0 completed | Learn |
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| Ordering Cleaved Fragments | 22 mins | 0 completed | Learn |
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Concept #1: HPLC
Concept #2: Normal-Phase HPLC
Concept #3: Reverse-Phase HPLC
Practice: What is the order of elution (first → last) of the following amino acids in reverse-phase HPLC: Ala, Arg, Leu.
Practice: What is the basis for the separation of proteins by the following techniques?
Concept #4: HPLC Chromatogram
Practice: In the following HPLC chromatogram, which amino acid was the third substance eluted from the column?
