Concept #1: Edman Degradation Reaction Efficiency
Concept #2: Calculating Cumulative Yield
Practice: Assuming 98% reaction efficiency, calculate the total cumulative yield of the correct PTH-amino acid at the 50th Edman degradation cycle.
Practice: A) A peptide with the primary structure Lys-Arg-Pro-Leu-Ile-Asp-Gly-Ala is sequenced by the Edman degradation procedure. If each Edman cycle is 93% efficient, what percentage of the PTH-amino acids in the fourth Edman cycle will be PTH-Leu?
B) What percentage of the PTH-amino acids in the eighth Edman cycle will be PTH-Ala?