Practice: Calculate the degree of inhibition of an inhibitor on the free enzyme if the [I] = 3 μM and the KI = 6 μM.
Concept #1: Degree of Inhibition
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Concept #2: α Measures Effects of Inhibition on Free Enzyme (E)
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Example #1: According to the data in the table below, which enzyme is affected most by the inhibitor?
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Practice: Calculate the degree of inhibition of an inhibitor on the free enzyme if the [I] = 3 μM and the KI = 6 μM.
Concept #3: α’ Measures Effects of Inhibition on ES-Complex
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Practice: Calculate the degree of inhibition of an inhibitor on the ES-complex if the [I] = 8 μM and the K’I = 0.03 mM.
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