Concept #1: Degree of Inhibition

Concept #2: α Measures Effects of Inhibition on Free Enzyme (E)

Example #1: According to the data in the table below, which enzyme is affected most by the inhibitor?

Practice: Calculate the degree of inhibition of an inhibitor on the free enzyme if the [I] = 3 μM and the KI = 6 μM.

Concept #3: α’ Measures Effects of Inhibition on ES-Complex

Practice: Calculate the degree of inhibition of an inhibitor on the ES-complex if the [I] = 8 μM and the K’I = 0.03 mM.