Clutch Prep is now a part of Pearson
Ch. 8 - Protein Function WorksheetSee all chapters
All Chapters
Ch. 1 - Introduction to Biochemistry
Ch. 2 - Water
Ch. 3 - Amino Acids
Ch. 4 - Protein Structure
Ch. 5 - Protein Techniques
Ch. 6 - Enzymes and Enzyme Kinetics
Ch. 7 - Enzyme Inhibition and Regulation
Ch. 8 - Protein Function
Ch. 9 - Carbohydrates
Ch. 10 - Lipids
Ch. 11 - Biological Membranes and Transport
Ch. 12 - Biosignaling
Clutch Review 1: Nucleic Acids, Lipids, & Membranes
Clutch Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway
Clutch Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism
Clutch Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation
Introduction to Protein-Ligand Interactions
Protein-Ligand Equilibrium Constants
Protein-Ligand Fractional Saturation
Myoglobin vs. Hemoglobin
Heme Prosthetic Group
Hemoglobin Cooperativity
Hill Equation
Hill Plot
Hemoglobin Binding in Tissues & Lungs
Hemoglobin Carbonation & Protonation
Bohr Effect
BPG Regulation of Hemoglobin
Fetal Hemoglobin
Sickle Cell Anemia
Chymotrypsin's Catalytic Mechanism
Glycogen Phosphorylase
Liver vs Muscle Glycogen Phosphorylase
Motor Proteins
Skeletal Muscle Anatomy
Skeletal Muscle Contraction

Concept #1: Chymotrypsin's Catalytic Mechanism

Concept #2: Acylation Phase of Chymotrypsin's Mechanism

Concept #3: Deacylation Phase of Chymotrypsin's Mechanism

Practice: Chymotrypsin’s acylation phase is below. The catalytic process of __________ is illustrated by arrow # ____ and the catalytic process of _________________________ is illustrated by arrow # ____.

Concept #4: Review of Chymotrypsin's Entire Catalytic Mechanism

Practice: In the mechanism of chymotrypsin, serine-195:

Practice: Sequentially number the following steps of Chymotrypsin’s catalytic mechanism in the correct order from 1-8:

a) Released newly formed amine portion of the substrate diffuses away _______

b) His-57 deprotonates the Ser-195 hydroxyl group, generating a stronger nucleophile ______

c) Tetrahedral intermediate collapses & His-57 donates a H+ to N of scissile bond (cleaved peptide bond) ______

d) Via general-base-catalysis, His-57 deprotonates a water molecule, generating OH- ______

e) Tetrahedral intermediate collapses & His-57 donates a proton to Ser-195 (cleaving ester bond) _______

f) Released newly formed carboxylic acid portion of the substrate diffuses away & enzyme is restored _______

g) Hydroxide ion attacks the carbonyl group of the substrate, forming another tetrahedral intermediate ______

h) Nucleophilic Ser-195 attacks the carbonyl C of the substrate forming a tetrahedral intermediate______