Concept #1: Cyanogen Bromide (CNBr)
Practice: What fragments result from treating the following peptide with CNBr?
Concept #2: Hydrazine
Practice: Sketch the approximate result of the following reaction.
Concept #3: Beta-Mercaptoethanol + Iodoacetate
Practice: Which of the following statements about cystine is correct?
Concept #4: Recap of Chemical Bond Cleavage
Practice: Given the amino acid sequence DAHGHFLKAGMT, what reagent would cleave the last residue, T, from the peptide? That is, one of the products after treatment will be the free amino acid: T.
Practice: One method to prevent disulfide bond interference with protein sequencing procedures is: