Protein Purification
Protein Extraction
Differential Centrifugation
Salting Out
Column Chromatography
Ion-Exchange Chromatography
Anion-Exchange Chromatography
Size Exclusion Chromatography
Affinity Chromatography
Specific Activity
Native Gel Electrophoresis
SDS-PAGE Strategies
Isoelectric Focusing
Diagonal Electrophoresis
Mass Spectrometry
Mass Spectrum
Tandem Mass Spectrometry
Peptide Mass Fingerprinting
Overview of Direct Protein Sequencing
Amino Acid Hydrolysis
Chemical Cleavage of Bonds
Edman Degradation
Edman Degradation Sequenator and Sequencing Data Analysis
Edman Degradation Reaction Efficiency
Ordering Cleaved Fragments
Strategy for Ordering Cleaved Fragments
Indirect Protein Sequencing Via Geneomic Analyses

Concept #1: Cyanogen Bromide (CNBr)

Practice: What fragments result from treating the following peptide with CNBr? 

Practice: Sketch the approximate result of the following reaction.

Concept #3: Beta-Mercaptoethanol + Iodoacetate

Practice: Which of the following statements about cystine is correct?

Concept #4: Recap of Chemical Bond Cleavage

Practice: Given the amino acid sequence DAHGHFLKAGMT, what reagent would cleave the last residue, T, from the peptide? That is, one of the products after treatment will be the free amino acid: T.

Practice: One method to prevent disulfide bond interference with protein sequencing procedures is: