Ch. 12 - BiosignalingWorksheetSee all chapters
All Chapters
Ch. 1 - Introduction to Biochemistry
Ch. 2 - Water
Ch. 3 - Amino Acids
Ch. 4 - Protein Structure
Ch. 5 - Protein Techniques
Ch. 6 - Enzymes and Enzyme Kinetics
Ch. 7 - Enzyme Inhibition and Regulation
Ch. 8 - Protein Function
Ch. 9 - Carbohydrates
Ch. 10 - Lipids
Ch. 11 - Biological Membranes and Transport
Ch. 12 - Biosignaling
Clutch Review 1: Nucleic Acids, Lipids, & Membranes
Clutch Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway
Clutch Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism
Clutch Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation
Sections
Introduction to Biosignaling
G protein-Coupled Receptors
Stimulatory Adenylate Cyclase GPCR Signaling
cAMP & PKA
Inhibitory Adenylate Cyclase GPCR Signaling
Drugs & Toxins Affecting GPCR Signaling
Recap of Adenylate Cyclase GPCR Signaling
Phosphoinositide GPCR Signaling
PSP Secondary Messengers & PKC
Recap of Phosphoinositide Signaling
Receptor Tyrosine Kinases
Insulin
Insulin Receptor
Insulin Signaling on Glucose Metabolism
Recap Of Insulin Signaling in Glucose Metabolism
Insulin Signaling as a Growth Factor
Recap of Insulin Signaling As A Growth Factor
Recap of Insulin Signaling
Jak-Stat Signaling
Lipid Hormone Signaling
Summary of Biosignaling
Signaling Defects & Cancer

Concept #2: Inactivation of cAMP & PKA

Example #1: Order each of the steps in the activation of PKA (by numbering them 1-4 ; first step is already numbered for you) starting after adenylate cyclase activation.

Practice: Which of the following statements about protein kinase A (PKA) is false?

Concept #3: Inactivation of cAMP & PKA

Practice: Which of the following is/are associated with cAMP binding to cAMP-dependent protein kinase A (PKA)? 

I. cAMP binds to the regulatory subunits.
II. Tetrameric regulatory subunits and catalytic subunits dissociate.
III. Catalytic subunits phosphorylate proteins with specific Ser and Thr residues.
IV. cAMP is membrane bound via a phosphoinositol covalent attachment.

Practice: What is the function of a phosphatase?

Practice: What could be the result of a mutation in the R subunits of cAMP-dependent protein kinase A (PKA) that inhibits formation of the R2C2 protein complex?

Practice: The image below is a schematic representation of PKA activation from epinephrine binding. Based on the provided numbers in the diagram, how many subunits of catalytically active PKA will there be?