Concept #1: Calculating K_{m} with Algebra

Concept #2: Calculating K_{m} with Reaction Rates

Example #1: The following rate constants were measured for a simple enzyme-catalyzed reaction. Determine the K_{m}.

Practice: To determine the Km from a Lineweaver-Burk plot you would:

Practice: The V_{max} for an enzyme is 9 mg/min. Calculate the K_{m} if the [S] = 5 mM when the V_{0} = 3 mg/min.

Practice: Calculate the Km of an enzyme using Michaelis-Menten kinetics if the forward rate constant for ES formation is 4.3 x 10^{6} M^{-1}s^{-1}, the reverse rate constant for ES dissociation into E + S is 2.4 x 10^{2} s^{-1}, and the forward rate constant for ES dissociation into E + P is 1.2 x 10 ^{3} s^{-1}.