Calculating Km

Practice: To determine the Km from a Lineweaver-Burk plot you would:

Practice: The V_max for an enzyme is 9 mg/min. Calculate the K_m if the [S] = 5 mM when the V₀ = 3 mg/min.

Practice: Calculate the Km of an enzyme using Michaelis-Menten kinetics if the forward rate constant for ES formation is 4.3 x 10⁶ M^-1s^-1, the reverse rate constant for ES dissociation into E + S is 2.4 x 10² s^-1, and the forward rate constant for ES dissociation into E + P is 1.2 x 10 ³ s^-1.