Concept #1: Calculating Km with Algebra
Concept #2: Calculating Km with Reaction Rates
Example #1: The following rate constants were measured for a simple enzyme-catalyzed reaction. Determine the Km.
Practice: To determine the Km from a Lineweaver-Burk plot you would:
Practice: The Vmax for an enzyme is 9 mg/min. Calculate the Km if the [S] = 5 mM when the V0 = 3 mg/min.
Practice: Calculate the Km of an enzyme using Michaelis-Menten kinetics if the forward rate constant for ES formation is 4.3 x 106 M-1s-1, the reverse rate constant for ES dissociation into E + S is 2.4 x 102 s-1, and the forward rate constant for ES dissociation into E + P is 1.2 x 10 3 s-1.