Clutch Prep is now a part of Pearson
Ch. 8 - Protein Function WorksheetSee all chapters
All Chapters
Ch. 1 - Introduction to Biochemistry
Ch. 2 - Water
Ch. 3 - Amino Acids
Ch. 4 - Protein Structure
Ch. 5 - Protein Techniques
Ch. 6 - Enzymes and Enzyme Kinetics
Ch. 7 - Enzyme Inhibition and Regulation
Ch. 8 - Protein Function
Ch. 9 - Carbohydrates
Ch. 10 - Lipids
Ch. 11 - Biological Membranes and Transport
Ch. 12 - Biosignaling
Clutch Review 1: Nucleic Acids, Lipids, & Membranes
Clutch Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway
Clutch Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism
Clutch Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation
Introduction to Protein-Ligand Interactions
Protein-Ligand Equilibrium Constants
Protein-Ligand Fractional Saturation
Myoglobin vs. Hemoglobin
Heme Prosthetic Group
Hemoglobin Cooperativity
Hill Equation
Hill Plot
Hemoglobin Binding in Tissues & Lungs
Hemoglobin Carbonation & Protonation
Bohr Effect
BPG Regulation of Hemoglobin
Fetal Hemoglobin
Sickle Cell Anemia
Chymotrypsin's Catalytic Mechanism
Glycogen Phosphorylase
Liver vs Muscle Glycogen Phosphorylase
Motor Proteins
Skeletal Muscle Anatomy
Skeletal Muscle Contraction

Concept #1: BPG Effect on Oxygen Binding

Concept #2: BPG Only Affects Hb in the Tissues

Concept #3: Physiological Regulation of [BPG]

Practice: In hemoglobin, the equilibrium transition from R state to T state is triggered by:

Practice: A 9-month old girl with hemolytic anemia is found to have a deficiency in the enzyme responsible for the conversion of molecule-B to pyruvate. Shown below is the oxygen saturation curve for hemoglobin in the erythrocytes of this patient (green curve), compared to the corresponding black curve in normal red blood cells. Which of the following is the most likely explanation for the observed oxygen saturation curve in this patient?

Practice: What is the effect of the following changes on the O 2 affinity of hemoglobin?

A) A drop in the pH of blood plasma from 7.4 to 7.2.

a) Lower the O2 Affinity.     b) Increase the O2 Affinity.

B) A decrease in the partial pressure of CO2 in the lungs from 6 kPa (holding one's breath) to 2 kPa (normal).

a) Lower the O2 Affinity.      b) Increase the O2 Affinity.

C) An increase in [BPG] from 5 mM (normal altitudes) to 8 mM (high altitudes).

a) Lower the O2 Affinity.     b) Increase the O2 Affinity.