Affinity Chromatography

Practice: In your own words, describe the principles involved in protein purification by affinity chromatography.

Practice: The target protein to be purified is likely eluted from the affinity chromatography column by _______________. Explain potential advantages & disadvantages of the elution strategies.

Practice: A biochemist is attempting to separate a DNA-binding protein (protein X) from other proteins in solution (proteins A, B & C). Consider the chart & answer the questions below about what type of technique is best for separation.

A. What type of chromatography is best for separating protein X from protein A? ___________________________

B. What type of chromatography is best for separating protein X from protein B? ___________________________

C. What type of chromatography is best for separating protein X from protein C? __________________________