2D-Electrophoresis

Practice: Use the results of the two-dimensional electrophoresis gel below to answer the following questions. 

A) Which protein or proteins have the highest pI value?

a. Protein a.                                    b. Proteins d & e.

b. Proteins b & c.

B) Which protein or proteins have the highest molecular weight?

a. Protein a.                                    c. Protein c.

b. Protein b.                                    d. Proteins d & e.

C) Which protein or proteins have identical molecular weights?

a. Proteins a & d.                           c. Proteins d & e.

b. Proteins b & c.                           d. None. Each has a unique weight.

Practice: Which of the following is true in 2D-electrophoresis?

Practice: An average protein will not be denatured by:

Practice: Sketch the result of 2D gel electrophoresis on the following four proteins (see chart) & label them clearly.