Ch.17 - Fundamentals of SpectrophotometryWorksheetSee all chapters
All Chapters
Ch.1 - Chemical Measurements
Ch.2 - Tools of the Trade
Ch.3 - Experimental Error
Ch.4 + 5 - Statistics, Quality Assurance and Calibration Methods
Ch.6 - Chemical Equilibrium
Ch.7 - Activity and the Systematic Treatment of Equilibrium
Ch.8 - Monoprotic Acid-Base Equilibria
Ch.9 - Polyprotic Acid-Base Equilibria
Ch.10 - Acid-Base Titrations
Ch.11 - EDTA Titrations
Ch.12 - Advanced Topics in Equilibrium
Ch.13 - Fundamentals of Electrochemistry
Ch.14 - Electrodes and Potentiometry
Ch.15 - Redox Titrations
Ch.16 - Electroanalytical Techniques
Ch.17 - Fundamentals of Spectrophotometry
BONUS: Chemical Kinetics
Properties of Light
Absorption of Light
Measuring Absorbance
Beer's Law
Instrument Components
Measuring Absorbance 

Example #1:

In UV-Vis spectroscopy, the absorbance represents the quantity of light that a sample absorbs, while the percent transmittance represents the amount of light that passes through a sample. Calculate the percent transmittance of a sample if its absorbance is measured as 0.419.


Example #2: If your sample has a percent transmittance of 39.1%, determine the absorbance of the compound. 

Practice: Identify the colors of a compound that absorbs green light, yellow-green light and blue light.